5 edition of PCR protocols found in the catalog.
Includes bibliographical references and index.
|Statement||edited by Daniel J. Park|
|Series||Springer protocols, Methods in molecular biology -- 687, Springer protocols, Methods in molecular biology (Clifton, N.J.) -- v. 687.|
|LC Classifications||QP606.D46 P3595 2011|
|The Physical Object|
|Pagination||xi, 348 p. :|
|Number of Pages||348|
|LC Control Number||2011282439|
Given that many NPs are capable of enhancing PCR efficiency, it is clear that there is likely to be great potential for nanoPCR technology improvements and product development. It was originally described in a study to verify the presence of the microbe Yersinia pestis in dental samples obtained from 14th Century graves of people supposedly killed by plague during the medieval Black Death epidemic. Diseases such as pertussis or whooping cough are cause by the bacteria Bordetella pertussis. Medical and diagnostic applications[ edit ] Prospective parents can be tested for being genetic carriersor their children might be tested for actually being affected by a disease. Hence the name 'digital PCR'. In this step, the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding free dNTPs from the reaction mixture that are complementary to the template in the 5'-to-3' direction, condensing the 5'- phosphate group of the dNTPs with the 3'- hydroxy group at the end of the nascent elongating DNA strand.
Some PCR 'fingerprints' methods have high discriminative power and can be used to identify genetic relationships between individuals, such as parent-child or between siblings, and are used in paternity testing Fig. The book should prove to be a valuable tool to all those interested in PCR technology. The child has inherited some, but not all of the fingerprint of each of its parents, giving it a new, unique fingerprint. It is used in sequencing and hybridization probing where amplification of only one of the two complementary strands is required.
Denton and H. His interest in chemistry started when he learned how to chemically synthesize and build solid-state fuel propulsion rockets as a high school student during the s. These mutations can be chosen in order to understand how proteins accomplish their functions, and to change or improve protein function. Cutting-edge and highly practical, PCR Protocols, Second Edition provides both novice and experienced investigators with an up-to-date compendium of powerful PCR methods for easy reference and consultation in the day-to-day performance of PCR-based experimentation, one that will enhance understanding of PCR, satisfy current needs, and point to powerful future applications. Friedman, N. Kellogg and S.
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Davis, K. His family had a background in farming in this rural area. Plateau: No more product accumulates due to exhaustion of reagents and enzyme. Krummel, DNase I Footprinting.
Mullis,The unusual origin of the polymerase chain reaction. The breadth of the applications and methodology described in this book should give even the novice the confidence and skill to make PCR work Cross, N.
The first category is called variable number tandem repeats VNTRwhich are 10— base pairs long and the second category is called short tandem repeats STR and these consist of repeated 2—10 base pair sections. The precise time required for elongation depends both on the DNA polymerase used and on the length of the DNA target region to amplify.
Examples of Standard Operating Procedures as used in individual specialist laboratories and an outline of training materials necessary for PCR technology transfer are presented. The book should prove to be a valuable tool to all those interested in PCR technology.
By analyzing several STRs for an individual, a set of alleles for each person will be found that statistically is likely to be unique. Holland and M. Diseases such as pertussis or whooping cough are cause by the bacteria Bordetella pertussis.
Similarly, unusual deletions, insertions, translocations, or inversions can be analyzed, all without having to wait or pay for the long and laborious processes of fertilization, embryogenesis, etc.
Gobind Khorana and Kjell Kleppe, a Norwegian scientist, authored a paper 17 years earlier describing a process they termed "repair replication" in the Journal of Molecular Biology.
Nobel laureate H. Kwon, and F. The earliest tests for infection relied on the presence of antibodies to the virus circulating in the bloodstream. There are, however, many problems associated with the transfer and particularly the application of this technology.
Detection of DNA using these methods can only be seen after the hybridization of probes with its complementary DNA takes place. Sarkar, D. This requirement makes it possible to delineate a specific region of template sequence that the researcher wants to amplify.
This bacteria is marked by a serious acute respiratory infection that affects various animals and humans and has led to the deaths of many young children. Further reading.
Baginski, A. PCR amplification under stringent conditions is much less efficient in the presence of a mismatch between template and primer, so successful amplification with an SNP-specific primer signals presence of the specific SNP in a sequence.
Perrin, and H. MSP using qPCR can also be performed to obtain quantitative rather than qualitative information about methylation. It is critical to determine a proper temperature for the annealing step because efficiency and specificity are strongly affected by the annealing temperature.This tool will generate PCR protocols for use with Optimase, the high-fidelity polymerase product from Transgenomic.
To generate a PCR protocol, enter your primers and anticipated PCR product length, and select a PCR protocol type. When you click on "Develop PCR protocol", this software will generate the appropriate PCR protocol. These range from basic beginners level PCR books to high-level books on PCR Troubleshooting and books aimed at advanced users on specialist PCR topics.
A huge range of PCR books is available and it is difficult to choose the right book. The following book reviews are a selection of published reviews on books about PCR. Feb 08, · texts All Books All Texts latest This Just In Smithsonian Libraries FEDLINK (US) Genealogy Lincoln Collection.
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PCR Protocols, Second Edition Item Preview. In its 3rd Edition, this book selects recently developed tools and tricks, for the value they add to established methods. The text describes core applications: PCR. Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications.
Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the Author: Roberto Biassoni.
Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study. Related Science Books: Reversible Deactivation Radical Polymerization.